等离子体活化水装置的开发与灭菌效果研究

论文总字数：31931字

摘 要

大气压冷等离子体在生物医学、环境保护等领域展现了广阔的应用前景，等离子体生物医学是当前等离子体科学的热点课题，包括癌症治疗、等离子体灭菌等。本文以沿面介质阻挡放电为原理开发一套等离子体活化水装置，并对活化水的杀菌效果进行检测和分析。该装置具有良好的应用前景，包括可用作制备漱口水以及其它用途的消毒水。

本文首先介绍了等离子体与水溶液相互作用的基本原理，阐述了活性粒子在水溶液中的关键作用。在此基础上，开发了一套等离子体活化水装置，此装置放电均匀稳定，具有较大处理面积，可以处理各种水溶液以制备等离子体活化水。

其次，本文研究了等离子体活化水的灭菌效果。试验中，以金黄色葡萄球菌作为灭活对象，对等离子体活化的不同浓度过氧化氢溶液进行了灭菌效果检测，结果显示，与医用双氧水和洗必泰相比，等离子体活化水具有显著更强的杀菌效果。

最后，本文通过仿真分析了等离子体活化水中主要活性粒子，结合实验研究，对等离子体活化水的灭菌机制进行了探讨，初步认为OONOOH在灭菌过程起着关键作用，它能够氧化细菌的过氧化氢酶来破坏细菌的防御机制。

关键词: 冷等离子体，沿面介质阻挡放电，过氧化氢溶液，金黄色葡萄球菌，OONOOH

Development of Plasma activated solution device and study the sterilization effect of plasma activated solution

Abstract

Atmospheric pressure cold plasma has showed the broad application prospects in the biomedical, environmental protection and other fields. Plasma Biomedicine is currently a hot topic in Plasma Science, including cancer treatment, Plasma sterilization. This paper developed a Plasma activated solution device according to the principle of Surface Dielectric Barrier Discharge, with this device we detected and analyzed the sterilization of Plasma activated solution. This device is of great application prospects as it produce mouthwash and disinfectants.

This paper introduces the basic principles of plasma interaction with the solution firstly, expounded the key effect of reactive species in solution. On the basis of above, we developed a Plasma activated solution device, this device discharge stably and has a large treatment area, which can be used to activate different kinds of solution.

Secondly, this paper studied the sterilization effect of plasma activated solution. During the experiment, we selected Staphylococcus aureus as sterilized objects, tested the sterilization effect of Plasma activated hydrogen peroxide solution with different concentration. The results show that compared with medical hydrogen peroxide and chlorhexidine, plasma activated aqueous solution had a significantly stronger bactericidal effect.

Finally, by the means of simulation, this paper analyzed the main reactive species in the Plasma activated solution, combined with experimental studies, we discussed the sterilization mechanism of Plasma activated solution, and preliminarily thought that OONOOH plays a key role in the sterilization process, it is capable of oxidizing bacteria hydrogen peroxide enzymes to destroy the bacteria's defense mechanism.

KEY WORDS: Cold plasma, Surface Dielectric Barrier Discharge, Hydrogen peroxide solution, Staphylococcus aureus, OONOOH

目 录

摘 要 I

Abstract II

第一章 绪论 1

1.1 大气压冷等离子体介绍 1

1.2 冷等离子体灭菌研究现状 3

1.3 本文研究的主要内容 4

第2章 等离子体与水溶液相互作用的基本原理 5

2.1 等离子体与水溶液相互作用的基本过程 5

2.2 等离子体与水溶液相互作用的实验诊断 5

2.3 等离子体与水溶液相互作用的仿真研究 7

2.4 本章小结 9

第3章 等离子体活化水装置的开发和检测 10

3.1 装置的制作和实验平台的搭建 10

3.2 实验材料的准备 13

3.2.1 处理液的准备 13

3.2.2 培养基和稀释液的准备 14

3.2.3 细菌的选择和培养 14

3.3 放电特性的检测 14

3.4 杀菌效果的检测 15

3.4.1 细菌的处理和活菌培养计数 15

3.4.2 杀菌效果的对比 16

3.4.3 中和剂的使用 18

3.5本章小结 18

第4章 等离子体活化水灭菌效果机理研究 19

4.1 等离子体活化水灭菌效果的影响因素 19

4.2等离子体活化水中活性粒子的仿真分析 20

4.3 等离子体活化水灭菌效果的机理分析 23

4.4 本章小结 24

结 论 26

展 望 27

致 谢 28

参考文献（References） 29

第一章 绪论

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