弱光环境下高湿胁迫对设施葡萄生理特性的影响及评价

论文总字数：17882字

目 录

摘要 1

Abstract 2

1材料与方法 3

1.1试验材料 3

1.2试验设置 3

1.3叶绿素测定 4

1.4保护酶测定 4

1.5数据处理 4

2讨论与分析 4

2.1弱光环境下，高湿胁迫对葡萄最大净光合速率的影响 4

2.2弱光环境下，高湿胁迫对葡萄叶绿素的影响 5

2.3弱光环境下，高湿胁迫对葡萄酶的影响 8

2.3.1高湿胁迫葡萄叶片MDA含量的影响 8

2.3.2高湿胁迫葡萄叶片CAT活性的影响 9

2.3.3高湿胁迫对葡萄叶片POD活性的影响 9

2.3.4高湿胁迫对葡萄叶片SOD活性的影响 11

2.4综合指标分析 11

3结论与讨论 13

参考文献 14

致谢 16

弱光环境下高湿对设施葡萄生理特性的影响及评价

李宝华

, China

Abstract: In this experiment, the “Reddish” grape variety was used as a test material, and a controlled test of high-humidity treatment of the test materials by various stress days was designed through an artificial climatic box, and (due to different humidity stress tests and recovery tests in a low-light environment), the grape leaves were measured. The maximum net photosynthetic rate, relative chlorophyll content, and protective enzyme content were studied to investigate the effects of high humidity stress on grape senescence and photosynthetic characteristics under weak light conditions. The results of the experiment showed that: (1) Low light conditions, high humidity stress on the facilities The photosynthetic characteristics of the grapes were inhibited. With the increase of high humidity, the contents of chlorophyll a and chlorophyll b showed a decreasing trend, and the chlorophyll a/b showed a wavy upward trend. (2) High-humidity stress accelerates senescence, and with the increase of high humidity, protective enzymes: catalase (CAT) activity, malondialdehyde (MDA) content, peroxidase (POD), superoxide dismutase ( SOD) content increased. (3) The higher the degree of high-humidity stress was, the longer the grape plants recovered to a level comparable to that of the control CK after resuming treatment; severe high-humidity stress was difficult to return to the control level. (4) With the increase of the degree of high humidity stress and the prolongation of time, the damage of grape victims is more serious, 75% of humidity has no significant effect on grapes; 85% of high humidity has caused damage to grape plants, and 95% of high humidity damages grape plants. The most serious. Integrating the recovery status of grape after different humidity stress and evaluating the comprehensive index SI value, the low-light and high-humidity disasters of facility grapes were classified into: non-hazard level (75% humidity 3~9d, 85% humidity 3~6d), mild Disaster (75% humidity 9~12d, 95% humidity 3d), moderate disaster (75% humidity 15d, 85% humidity 9~12d, 95% humidity 6d), severe disaster (85% humidity 15d, 95% humidity 9~ 15d).

Key words: Facilities grape; maximum net photosynthetic rate; protective enzyme; chlorophyll; malondialdehyde; membership function

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