不同CO2浓度升高水平对粳稻荧光特征和LAI的影响

论文总字数：26105字

目 录

1 引言 1

2 材料与方法 2

2.1 试验地点概况 2

2.2试验方法 2

2.2.1试验设计 2

2.2.2供试材料与田间管理............................................ 2

2.3测定项目与方法 3

2.3.1叶绿素含量的测定 3

2.3.2快速叶绿素荧光诱导动力学参数的测定 3

2.3.3叶面积指数的测定 3

2.4数据处理与统计分析 3

3结果与分析 3

3.1不同浓度CO₂升高条件对粳稻荧光参数的影响 3

3.1.1叶片快速叶绿素荧光诱导曲线的变化特征 3

3.1.2叶片PSII供体侧和受体侧的荧光特征 4

3.1.3叶片PSII反应中心的荧光特征 6

3.2不同浓度CO₂升高条件对粳稻叶面积指数的影响 9

4讨论 10

4.1不同浓度CO₂升高条件对粳稻荧光参数的影响 10

4.1.1叶片叶绿素荧光诱导曲线的变化特征 10

4.1.2叶片PSII供体侧和受体侧的荧光特征 11

4.1.3叶片PSII反应中心的荧光特征 11

4.2不同浓度CO₂升高条件对粳稻叶面积指数的影响 12

5 结论 12

参考文献 13

致谢 16

不同CO₂浓度升高水平对粳稻荧光特征和LAI的影响

王艺杰

，China

Abstract: Through the field open top chamber (OTC) test,an automatic CO₂ concentration control system was used to study the effects of different CO₂ concentrations on the photosynthetic characteristics of rice. CO₂ concentration was set at 3 levels: background atmospheric CO₂ concentration was used as control (CK),and 80 ppm (T₁) and 200 ppm (T₂) were increased on the basis of CK. The fast chlorophyll fluorescence-inducing kinetics and leaf area index (LAI) of Japonica Rice were measured in the key growth stages of Japonica Rice using the plant efficiency analyzer (Handy PEA) and the plant canopy analyzer LAI-2000. The results showed that: (1)There was no significant difference in leaf area index between CK and T₂ at the wax ripening stage. In the other growth stages, the CK and T₂ treatments achieved significant differences. However, the CK and T₁ treatments had no significant difference besides the flowering stage and the wax ripening stage. The CK and T₁ treatments also reached significant differences in the other growth stages; however, there was no significant difference in T₁ treatment and T₂ treatment during the entire growth period. In the comparison between T₁ treatment and T₂ treatment, T₂ treatment has a greater increase in leaf area index of Japonica Rice. (2) The three fluorescence parameters Sm, φ_Eo, and ψ_o of the donor side and receptor side of PSII increased under T₁ and T₂ treatment in some growth periods, and the increase of the three fluorescence parameters under T₁ treatment was greater than that of T₂ treatment. The rest of the growth period remains basically unchanged. (3) In the T₁ and T₂ treatment, the fluorescence parameters of the PSII reaction center were not different from those of CK except that the T₁ and T₂ were more than CK at the filling stage; the parameters in other growth stages were less than CK or no difference with CK. In general, different CO₂ concentration raising conditions will decrease the fluorescence parameters of the PSII reaction center during most growth periods of Japonica Rice. However, the growth period with the most significant difference in photochemical efficiency was T₁gt;T₂gt;CK. The increase of CO₂ concentration resulted in a significant increase in leaf area index. The chlorophyll fluorescence parameters of the donor and recipient sides of PSII increased. Although the chlorophyll fluorescence parameters of the PSII reaction center were unchanged or decreased, the maximum photochemical efficiency remained or increased.

Key words：Japonica Rice；CO₂ enrichment；PSII；chlorophyll fluorescence；leaf area index

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