论文总字数：27105字

摘 要

 随着我国老年人口增多，如何有效的预防并治疗骨质疏松等老年疾病成了社会关注的热点问题。催产素主要由下丘脑室旁核分泌，在分娩时有促进子宫收缩和在哺乳时调节排乳的作用，近年发现它还参与调节机体的骨代谢过程。研究表明磁性纳米颗粒在磁场中具有磁热效应，同时还可以调节细胞的增殖和分化。此外，磁场对机体的神经内分泌系统也具有调节作用。

本课题在小鼠室旁核注射磁性纳米颗粒，然后通过外加旋转磁场对小鼠进行刺激，检测小鼠血液中催产素的含量发现小鼠在磁场刺激后血液中催产素的含量有所增加。说明外加磁场的刺激会影响小鼠催产素的分泌水平。随后改变单一实验条件，通过旋转磁场刺激后血清中催产素的含量变化来选择最佳条件。通过比较注射磁性氧化铁纳米颗粒和金纳米颗粒以及对照组的小鼠，发现催产素分泌的变化主要是由磁性纳米颗粒感应磁场效应引起的。随后又依次筛选了磁场作用时间、注射磁性纳米颗粒的注射量和浓度，得到磁场作用的最佳时长为60min磁性纳米颗粒最佳注射量为0.2ul，最佳注射浓度为2mg/ml。

研究结果表明，磁场刺激可以调节机体催产素的分泌活动，而催产素与骨质疏松有一定的联系。探究磁场作用对催产素分泌水平的影响有望为缓解治疗骨质疏松提供一个良好对策。

关键词：催产素；磁性纳米颗粒；磁场；骨代谢；神经内分泌

Abstract

With the increase of the elderly population in China, how to effectively prevent and treat osteoporosis and other elderly diseases has become a hot issue of social concern. Oxytocin is mainly secreted by the paraventricular nucleus of the hypothalamus. Oxytocin has the function of promoting uterine contraction and adjusting breast milk during breastfeeding. In recent years, Oxytocin has been found to regulate bone metabolism in the body. Studies have shown that magnetic nanoparticles have magnetic properties in magnetic fields. And the thermal effect also regulates cell proliferation and differentiation. In addition, the magnetic field also has a regulatory effect on the neuroendocrine system of the body.

Injected magnetic nanoparticles in the paraventricular nucleus of mice, and then stimulated mice by applying a rotating magnetic field. Detect the concentration of oxytocin in mice blood and it was found that the content of oxytocin in the blood of mice increased after magnetic stimulation. It shows that the effect of the applied magnetic field will promote the secretion level of mouse oxytocin. Afterwards, the single experimental conditions were changed and the optimal conditions were selected by changing the content of oxytocin in the serum after stimulation with a rotating magnetic field. By comparing the magnetic nanoparticles and the Gold nanoparticles with the control mice, it was found that the changes in secretion of oxytocin were mainly caused by the common effect of magnetic particle injection and magnetic field.. Other conditions were also screened, such as the time of the magnetic field, injection volume and concentration of injected magnetic nanoparticles. It was found that the optimal duration of the magnetic field was 60 minutes, the optimal injection volume of the magnetic nanoparticles was 0.2 ul, and the optimal injection concentration was 2 mg/ml.

The results of the study showed that magnetic stimulation can regulate the secretion of oxytocin in the body, and oxytocin has a certain relationship with osteoporosis. Exploring the effect of magnetic field on the secretion of oxytocin is expected to provide a good solution for the treatment of osteoporosis.

KEY WORDS: Oxytocin; magnetic nanoparticles; magnetic field; bone metabolism; neuroendocrine

目录

摘要 I

Abstract II

第一章 绪论 1

1.1 催产素及催产素受体 1

1.1.1 催产素的合成与分泌 1

1.1.2 催产素受体及功能 1

1.1.3 催产素对骨代谢节作用的研究进展 1

1.2 磁性纳米颗粒 3

1.2.1 磁性纳米颗粒的特性 3

1.2.2 磁性纳米颗粒的制备方法 3

1.2.3 磁性纳米颗粒的应用 3

1.2.4 磁性纳米颗粒对前成骨细胞的影响 4

1.3 磁刺激对神经内分泌系统的影响 5

1.4 研究内容及意义 6

第二章 实验设备及试验方法 7

2.1 实验材料 7

2.1.1 实验小鼠 7

2.1.2 实验试剂 7

2.1.3 主要溶液配制方法 7

2.1.4 实验仪器 7

2.2 试验方法 7

2.2.1 鼠室旁核脑定位注射磁性纳米颗粒 7

2.2.2 旋转磁场作用室旁核注射小鼠 8

2.2.3 眼球取血做酶联免疫吸附实验检测催产素及骨代谢指标 8

第三章 实验结果与讨论 9

3.1 室旁核磁刺激对催产素分泌及骨代谢的影响 9

3.2 磁性纳米颗粒及磁场对催产素分泌的影响 10

3.3 旋转磁场作用的时间对催产素分泌的影响 12

3.4 磁性纳米颗粒的注射量对催产素分泌的影响 13

3.5 磁性纳米颗粒的注射浓度对催产素分泌的影响 15

第四章 结论 17

致谢 19

参考文献 20

绪论

我国已经步入老龄化，老年人人数有明显的上升趋势。骨质疏松症作为常见老年病，越来越影响人们的生活质量。因此近些年对骨质疏松症等老年疾病的研究原来越受关注。催产素主要由下丘脑室旁核分泌，近期研究发现它除了分娩时促进子宫收缩和在哺乳时调节排乳，还对机体骨代谢过程中也有调节作用。

同时，已有研究表明外源性电磁场对骨和软骨都有治愈作用，并且该方法在缓解与改善患者骨质疏松症症状也是有效的。机体的神经内分泌系统会受到磁场作用的影响，而催产素主要是神经内分泌系统合成分泌，因此推测磁场可能会通过刺激室旁核从而对催产素起到调节作用。而且不同的刺激条件可能引起的变化也不相同。

催产素及催产素受体

催产素的合成与分泌

催产素（Oxytocin OT）主要是由催产素神经元合成并分泌的，他的前体是催产素-神经垂体素复合体组成的一种颗粒物质颗粒。催产素-神经垂体素复合体经催产素神经元合成后，会在细胞内沿着轴突运输，在运输的过程中，轴突内的酶会将这种复合体裂解为催产素和神经垂体素Ⅰ。在轴突末梢，催产素和神经垂体素Ⅰ会分别以分泌小泡的形式存在，以便在收到刺激时释放。当神经元受到外界刺激产生动作电位后，细胞膜的通透性会发生改变，Na 与Ca2 会通过细胞膜进入细胞，然后激活一系列的信号转导机制，最后分泌催产素。

剩余内容已隐藏，请支付后下载全文，论文总字数：27105字