论文总字数:8146字
摘 要
本实验研究了饥饿对泥鳅生化组成的影响,实验测定了饥饿时间0d、14d条件下泥鳅肌肉的水分、总糖、总脂和蛋白质含量。结果表明:饥饿0d、14d的泥鳅肌肉含水量分别为为60.50%、63.10%,两组泥鳅含水量差异均不显著(P﹥0.05);饥饿过程中泥鳅肌肉含糖量呈上升趋势,饥饿0d为1.28%,饥饿14d后上升为2.81%,两组泥鳅总糖含量差异显著(P<0.05);总脂和蛋白质相对含量都是先上升,后下降,总脂饥饿0d、14d后含量分别为10.41%、7.13%,蛋白质饥饿0d、14d后含量分别为62.17%、47.32%,两组泥鳅的总脂和蛋白质含量均存在显著性差异(P<0.05)。关键词:饥饿,泥鳅,生化组成
Effects of Hunger on Muscle Contents of Moisture, Protein ,Total sugar and Lipid of Misgurnus anguillicaudatus.
(Huaiyin Normal University ,School of Life Science, XuYunxia 191101076)
Abstract: The study was conducted to determine the effect of hunger on the biochemical compositon of Misgurnus anguillicaudatus. Experiments were divided into two groups, hungering for 0d and 14d respectively, and the determination of the content of the muscle from Misgurnus anguillicaudatus, the content of the moisture of musle ,lipid, protein from which was in hungry of 0d and 14d. The results showed that along with the extension of hunger, the content of the moisture of muscle which was in hungry of 0d and 14d were 60.50%, 63.10% respectively. There were no significant differences(P﹥0.05)all of the two groups. The content of the saccharide of the muscle rised from 1.28% to 2.81% after 14d. There were significant differences all of the two groups(P<0.05). The content of the lipid of muscle which was in hungry of 0d and 14d were 10.41%,7.13%, and the content of the protein from muscle of Misgurnus anguillicaudatus which was in hungry of 0d and 14d were 62.17%,47.32% respectively.There were significant differences of the content of lipid and protein all of the two groups(P<0.05).
Keywords: Hunger, Misgur nus anguillicaudatus, Chemical composition
目录
1 引言 5
2 材料来源及实验设计 5
2.1泥鳅的来源 5
2.2实验设计 5
2.3 样品测定 6
2.3.1 含水量测定 6
2.3.2 总糖的测定 6
2.3.3 总脂的测定 6
2.3.4 蛋白质的测定 6
3.计算与数据分析 6
3.1饥饿对泥鳅肌肉含水量的影响 6
3.2饥饿对泥鳅肌肉总糖的影响 7
3.3 饥饿对泥鳅总脂的影响 7
3.4饥饿对泥鳅蛋白质的影响 8
4 讨论与分析 8
参考文献 11
致谢 12
1.引言
泥鳅( Misgurnus anguillicaudatus) 属鲤形目,鳅科,广泛分布于我国南方各地的江河、湖泊、水库、池塘及沟渠中,北方不常见但也分布各地。泥鳅被称为“水中之参”,全年都可采收,夏季最多,泥鳅捕捉后,可鲜用或烘干用。该鱼生长快,食性杂,抗病力强,因其个体小且易得,经济效益高、饲养方法简易且花工较少等优点,所以已越来越受养殖户的欢迎,是一种十分适合养殖的经济鱼类,也是一种较理想的实验鱼材料。泥鳅味道鲜美,营养丰富,含蛋白质较高而脂肪较低,能降脂降压,即是美味佳肴又是大众食品,素有“天上的斑鸠,地下的泥鳅”和“水中人参”之美誉。美味又滋补,还易获得,价廉物美。随着人们生活水平的提高,人们对泥鳅的需求量日益递增,野生泥鳅的产量已远远不能满足市场需求。
许多鱼类都能忍受较长时间饥饿[1]。饥饿状态下,鱼类的代谢机能发生改变, 并通过利用鱼体自身的贮存物质如糖类、脂肪和蛋白质提供能量,但不同鱼类在饥饿状态下能源物质的贮存和利用有一定差异[2] 。本实验以泥鳅为实验对象,分别研究了不同饥饿时间下泥鳅肌肉中的水分、糖类、脂肪、蛋白质含量,旨在为生产上泥鳅的饲料配置提供理论支持。
2 材料来源及实验设计
2.1泥鳅的来源
实验泥鳅于2015年3月取自江苏省市场,平均体重24.40±4.10g。
2.2实验设计
将实验泥鳅取至实验室后暂养10d以适应实验室环境,然后开始实验。实验分为两组,分别为饥饿0d、14d,每组三个重复。实验期间禁止投饵,每天9:00捞出死泥鳅,并移走粪便,水族箱每天换水1/5,配以24小时充气,实验期间水温保持在25±1℃。实验开始时取0d组泥鳅,用吸水纸将泥鳅体表的水分吸干,分别量取体长,称取体重,人工去皮后称取肌肉重,并且记录数据,放入冷藏柜中备用,另外两组到取样时间时取样,方法同上。
2.3 样品测定
剩余内容已隐藏,请支付后下载全文,论文总字数:8146字
该课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找;