摘 要

关键词：苍术 苯酚-硫酸法 正交试验 HPLC-ELSD

Qualitative and Quantitative Studies of Oligosaccharides in Medicinal Plants

Abstract:Atractylodes rhizome as a commonly used traditional Chinese medicine, its pharmacological effects are very wide, and also very effective in clinical applications, but due to geographical differences, the active ingredients in different habitats are often very different. In this experiment, oligosaccharides from the leaves of different habitats were used as the research object, and the oligosaccharides in the herb were extracted by ethanol reflux method, and the oligosaccharide content was determined by the phenol-sulfuric acid method and high performance liquid chromatography to evaluate the origin of each region. The level of oligosaccharides in the herb. Through orthogonal experimental design, the extraction conditions were optimized to obtain the optimal extraction conditions for oligosaccharides. Since the oligosaccharides have no UV absorption, ELSD can be used as a detector of oligosaccharides. Therefore, HPLC-ELSD was used to analyze the extracted oligosaccharides and optimize the analysis conditions. The oligosaccharides collected from different habitats were extracted and analyzed using the optimized extraction and separation methods. The experimental results showed that the content of oligosaccharides from different origins varies greatly, and lays the foundation for quality control research of Atractylodes lancea. basis.

Keywords: Atractylodes phenol-sulfuric acid method orthogonal test evaporative light scattering detecto

目录

第一章 文献综述

1．寡糖概述……………………………………………………………………………1

1.1寡糖简介…………………………………………………………………………1

1.2 寡糖研究水平概述………………………………………………………………1

1.2.1国内外研究概况………………………………………………………………1

1.2.2药用植物中寡糖分离提取和优化概况………………………………………2

2.寡糖的检测方法……………………………………………………………………3

2.1蒸发光散射检测器………………………………………………………………3

2.2快速蛋白液相色谱………………………………………………………………4

2.3石墨化碳液体色谱-质谱法………………………………………………………4

2.4染色法……………………………………………………………………………4

2.5阴离子交换色谱法………………………………………………………………5

3.研究意义……………………………………………………………………………5

第二章 实验材料

2.1实验仪器和试剂…………………………………………………………………6

2.2实验药材…………………………………………………………………………7

第三章 实验方法

3.1 苍术中寡糖的提取………………………………………………………………8

3.1.1 苍术样品的制备………………………………………………………………8

3.1.2 提取溶剂的配制………………………………………………………………8

3.1.3 提取寡糖………………………………………………………………………8

3.1.4苯酚-硫酸法检测苍术寡糖的含量……………………………………………8

3.1.4.1标准曲线的制备……………………………………………………………8

3.1.4.2样品溶液的制备…………………………………………………………9

3.2 寡糖提取条件的优化…………………………………………………………9

3.2.1 反应溶剂的优化……………………………………………………………9

3.2.2 反应时间的优化……………………………………………………………10

3.2.3 反应温度的优化……………………………………………………………10

3.2.4 药液比的优化………………………………………………………………10

3.3 正交试验优化回流法提取苍术寡糖…………………………………………10

3.3.1单因素条件的筛选……………………………………………………………10

3.3.2正交设计优选条件……………………………………………………………12

3.3.3选取各单因素的最佳值为中间点……………………………………………12

3.3.4误差分析………………………………………………………………………12

3.4分析方法优化……………………………………………………………………12

1. 结果与讨论

4.1不同产地苍术寡糖含量…………………………………………………………14

4.2单因素控制苍术寡糖的提取……………………………………………………15

4.3正交法优化苍术寡糖的提取……………………………………………………18

4.4正交试验液相图…………………………………………………………………19

4.5分析条件的优化…………………………………………………………………24

第五章 结论与展望

5.1 结论……………………………………………………………………………26

5.2 展望……………………………………………………………………………26

参考文献……………………………………………………………………………27

致谢…………………………………………………………………………………29

第一章 文献综述

1．寡糖概述

1.1寡糖的简介

寡糖是指两个或者两个以上单糖单位以糖苷键相连形成的糖分子。大体上有以下几种方法获得低聚糖：微波固相合成方法、酸碱转化法、酶水解法等。

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